A reliable ultra-high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (UHPLC–ESI–MS/MS) method for the fast simultaneous determination of 13 nucleosides and nucleobases in Cordyceps sinensis (C.sinensis) with 2-chloroadenosine as internal standard was developed and validated.Samples were ultrasonically extracted in an ice bath thrice, and the optimum analyte separation was performed on an ACQUITY UPLCTM HSS C18 column (100 mm × 2.
STRATEGI AKTIF PASIF DALAM OPTIMALISASI PORTOFOLIO SAHAM INDEKS LQ-45
.
1 mm, 1.8 μm) with gradient elution.All targeted analytes were separated in 5.
5 min.Furthermore, all calibration curves showed good linear regression (r > 0.
Phytohormone and Putative Defense Gene Expression Differentiates the Response of ‘Hayward’ Kiwifruit to Psa and Pfm Infections
.9970) within the test ranges, and the limits of quantitation and detection of the 13 analytes were less than 150 and 75 ng/mL, respectively.
The relative standard deviations (RSDs) of intra- and inter-day precisions were <6.23%.Recoveries of the quantified analytes ranged within 85.
3%–117.3%, with RSD < 6.18%.
The developed UHPLC–ESI–MS/MS method was successfully applied to determine nucleosides and nucleobases in 11 batches of C.sinensis samples from different regions in China.The range for the total content in the analyzed samples was 1329–2057 µg/g.